An in vivo culture system for human embryos using an encapsulation technology: a pilot study
Identifieur interne : 001129 ( Main/Exploration ); précédent : 001128; suivant : 001130An in vivo culture system for human embryos using an encapsulation technology: a pilot study
Auteurs : C. Blockeel [Belgique] ; P. Mock [Suisse] ; G. Verheyen [Belgique] ; N. Bouche [Suisse] ; Ph. Le Goff [Suisse] ; Y. Heyman [France] ; C. Wrenzycki [Allemagne] ; K. Hffmann [Allemagne] ; H. Niemann [Allemagne] ; P. Haentjens [Belgique] ; M. J. De Los Santos [Espagne] ; M. Fernandez-Sanchez [Espagne] ; M. Velasco [Suisse] ; P. Aebischer [Suisse] ; P. Devroey [Belgique] ; C. Simn [Espagne]Source :
- Human Reproduction [ 0268-1161 ] ; 2009-04.
Abstract
BACKGROUND Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS The IUCS device comprised a perforated silicone hollow tube. The study included 13 patients (<36 years) undergoing a first intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in three groups. In Group 1, 12 h after ICSI, sibling oocytes were assigned to IUCS or conventional in vitro culture. The device was retrieved on Day 1, and all zygotes were cultured in vitro till Day 5. In Group 2, fertilized oocytes were assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for blastomere biopsy and fluorescence in situ hybridization (FISH) and cultured until Day 5. The highest quality blastocysts were transferred on Day 5. RESULTS Fertilization and embryo development were comparable in the in vitro and IUCS arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in Group 3 revealed more normal embryos using the IUCS (P 0.049). Three clinical pregnancies and live births were obtained: two from the IUCS arm and one from the in vitro arm. CONCLUSIONS Our pilot study shows that this new IUCS appears to be feasible and safe, supporting normal fertilization, embryo development and normal chromosomal segregation. Furthermore, live births are possible after the transient presence of a silicone device in the uterus.Clinicaltrials.gov: NCT00480103.
Url:
DOI: 10.1093/humrep/dep005
Affiliations:
- Allemagne, Belgique, Espagne, France, Suisse
- Basse-Saxe, Canton de Genève, Canton de Vaud, Région de Bruxelles-Capitale
- Bruxelles, Genève, Hanovre, Lausanne
- Université de Genève
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000A41
- to stream Istex, to step Curation: 000A40
- to stream Istex, to step Checkpoint: 000C38
- to stream Main, to step Merge: 001131
- to stream Main, to step Curation: 001129
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>An in vivo culture system for human embryos using an encapsulation technology: a pilot study</title><author><name sortKey="Blockeel, C" sort="Blockeel, C" uniqKey="Blockeel C" first="C." last="Blockeel">C. Blockeel</name></author><author><name sortKey="Mock, P" sort="Mock, P" uniqKey="Mock P" first="P." last="Mock">P. Mock</name></author><author><name sortKey="Verheyen, G" sort="Verheyen, G" uniqKey="Verheyen G" first="G." last="Verheyen">G. Verheyen</name></author><author><name sortKey="Bouche, N" sort="Bouche, N" uniqKey="Bouche N" first="N." last="Bouche">N. Bouche</name></author><author><name sortKey="Le Goff, Ph" sort="Le Goff, Ph" uniqKey="Le Goff P" first="Ph." last="Le Goff">Ph. Le Goff</name></author><author><name sortKey="Heyman, Y" sort="Heyman, Y" uniqKey="Heyman Y" first="Y." last="Heyman">Y. Heyman</name></author><author><name sortKey="Wrenzycki, C" sort="Wrenzycki, C" uniqKey="Wrenzycki C" first="C." last="Wrenzycki">C. Wrenzycki</name></author><author><name sortKey="Hffmann, K" sort="Hffmann, K" uniqKey="Hffmann K" first="K." last="Hffmann">K. Hffmann</name></author><author><name sortKey="Niemann, H" sort="Niemann, H" uniqKey="Niemann H" first="H." last="Niemann">H. Niemann</name></author><author><name sortKey="Haentjens, P" sort="Haentjens, P" uniqKey="Haentjens P" first="P." last="Haentjens">P. Haentjens</name></author><author><name sortKey="De Los Santos, M J" sort="De Los Santos, M J" uniqKey="De Los Santos M" first="M. J." last="De Los Santos">M. J. De Los Santos</name></author><author><name sortKey="Fernandez Sanchez, M" sort="Fernandez Sanchez, M" uniqKey="Fernandez Sanchez M" first="M." last="Fernandez-Sanchez">M. Fernandez-Sanchez</name></author><author><name sortKey="Velasco, M" sort="Velasco, M" uniqKey="Velasco M" first="M." last="Velasco">M. Velasco</name></author><author><name sortKey="Aebischer, P" sort="Aebischer, P" uniqKey="Aebischer P" first="P." last="Aebischer">P. Aebischer</name></author><author><name sortKey="Devroey, P" sort="Devroey, P" uniqKey="Devroey P" first="P." last="Devroey">P. Devroey</name></author><author><name sortKey="Simn, C" sort="Simn, C" uniqKey="Simn C" first="C." last="Simn">C. Simn</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:033DA12265BEEFC4242E6536902363F8C6BE7EA5</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1093/humrep/dep005</idno><idno type="url">https://api.istex.fr/document/033DA12265BEEFC4242E6536902363F8C6BE7EA5/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A41</idno><idno type="wicri:Area/Istex/Curation">000A40</idno><idno type="wicri:Area/Istex/Checkpoint">000C38</idno><idno type="wicri:doubleKey">0268-1161:2009:Blockeel C:an:in:vivo</idno><idno type="wicri:Area/Main/Merge">001131</idno><idno type="wicri:Area/Main/Curation">001129</idno><idno type="wicri:Area/Main/Exploration">001129</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">An in vivo culture system for human embryos using an encapsulation technology: a pilot study</title><author><name sortKey="Blockeel, C" sort="Blockeel, C" uniqKey="Blockeel C" first="C." last="Blockeel">C. Blockeel</name><affiliation wicri:level="3"><country xml:lang="fr">Belgique</country><wicri:regionArea>Centre for Reproductive Medicine, UZ Brussel, Brussels</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName></affiliation></author><author><name sortKey="Mock, P" sort="Mock, P" uniqKey="Mock P" first="P." last="Mock">P. Mock</name><affiliation wicri:level="3"><country xml:lang="fr">Suisse</country><wicri:regionArea>Anecova Inc., Lausanne</wicri:regionArea><placeName><settlement type="city">Lausanne</settlement><region nuts="3" type="region">Canton de Vaud</region></placeName></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Suisse</country><wicri:regionArea>University of Geneva, Geneva</wicri:regionArea><orgName type="university">Université de Genève</orgName><placeName><settlement type="city">Genève</settlement><region nuts="3" type="region">Canton de Genève</region></placeName></affiliation></author><author><name sortKey="Verheyen, G" sort="Verheyen, G" uniqKey="Verheyen G" first="G." last="Verheyen">G. Verheyen</name><affiliation wicri:level="3"><country xml:lang="fr">Belgique</country><wicri:regionArea>Centre for Reproductive Medicine, UZ Brussel, Brussels</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName></affiliation></author><author><name sortKey="Bouche, N" sort="Bouche, N" uniqKey="Bouche N" first="N." last="Bouche">N. Bouche</name><affiliation wicri:level="3"><country xml:lang="fr">Suisse</country><wicri:regionArea>Anecova Inc., Lausanne</wicri:regionArea><placeName><settlement type="city">Lausanne</settlement><region nuts="3" type="region">Canton de Vaud</region></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">Suisse</country><wicri:regionArea>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne</wicri:regionArea><placeName><settlement type="city">Lausanne</settlement><region nuts="3" type="region">Canton de Vaud</region></placeName></affiliation></author><author><name sortKey="Le Goff, Ph" sort="Le Goff, Ph" uniqKey="Le Goff P" first="Ph." last="Le Goff">Ph. Le Goff</name><affiliation wicri:level="3"><country xml:lang="fr">Suisse</country><wicri:regionArea>Anecova Inc., Lausanne</wicri:regionArea><placeName><settlement type="city">Lausanne</settlement><region nuts="3" type="region">Canton de Vaud</region></placeName></affiliation></author><author><name sortKey="Heyman, Y" sort="Heyman, Y" uniqKey="Heyman Y" first="Y." last="Heyman">Y. Heyman</name><affiliation wicri:level="1"><country xml:lang="fr">France</country><wicri:regionArea>Institut National de Recherche Agronomique, INRA, Jouy en Josas</wicri:regionArea><wicri:noRegion>Jouy en Josas</wicri:noRegion><wicri:noRegion>Jouy en Josas</wicri:noRegion></affiliation></author><author><name sortKey="Wrenzycki, C" sort="Wrenzycki, C" uniqKey="Wrenzycki C" first="C." last="Wrenzycki">C. Wrenzycki</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Hffmann, K" sort="Hffmann, K" uniqKey="Hffmann K" first="K." last="Hffmann">K. Hffmann</name><affiliation wicri:level="3"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover</wicri:regionArea><placeName><region type="land" nuts="2">Basse-Saxe</region><settlement type="city">Hanovre</settlement></placeName></affiliation></author><author><name sortKey="Niemann, H" sort="Niemann, H" uniqKey="Niemann H" first="H." last="Niemann">H. Niemann</name><affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institut fr Nutztiergenetik, FLI, Mariensee, Neustadt</wicri:regionArea><wicri:noRegion>Neustadt</wicri:noRegion><wicri:noRegion>Neustadt</wicri:noRegion></affiliation></author><author><name sortKey="Haentjens, P" sort="Haentjens, P" uniqKey="Haentjens P" first="P." last="Haentjens">P. Haentjens</name><affiliation wicri:level="3"><country xml:lang="fr">Belgique</country><wicri:regionArea>Centre for Outcomes Research and Laboratory for Experimental Surgery, UZ Brussel, Brussels</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName></affiliation></author><author><name sortKey="De Los Santos, M J" sort="De Los Santos, M J" uniqKey="De Los Santos M" first="M. J." last="De Los Santos">M. J. De Los Santos</name><affiliation wicri:level="1"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Valenciano de Infertilidad (IVI), Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Fernandez Sanchez, M" sort="Fernandez Sanchez, M" uniqKey="Fernandez Sanchez M" first="M." last="Fernandez-Sanchez">M. Fernandez-Sanchez</name><affiliation wicri:level="1"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Valenciano de Infertilidad (IVI), Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Velasco, M" sort="Velasco, M" uniqKey="Velasco M" first="M." last="Velasco">M. Velasco</name><affiliation wicri:level="3"><country xml:lang="fr">Suisse</country><wicri:regionArea>Anecova Inc., Lausanne</wicri:regionArea><placeName><settlement type="city">Lausanne</settlement><region nuts="3" type="region">Canton de Vaud</region></placeName></affiliation></author><author><name sortKey="Aebischer, P" sort="Aebischer, P" uniqKey="Aebischer P" first="P." last="Aebischer">P. Aebischer</name><affiliation wicri:level="3"><country xml:lang="fr">Suisse</country><wicri:regionArea>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne</wicri:regionArea><placeName><settlement type="city">Lausanne</settlement><region nuts="3" type="region">Canton de Vaud</region></placeName></affiliation></author><author><name sortKey="Devroey, P" sort="Devroey, P" uniqKey="Devroey P" first="P." last="Devroey">P. Devroey</name><affiliation wicri:level="3"><country xml:lang="fr">Belgique</country><wicri:regionArea>Centre for Reproductive Medicine, UZ Brussel, Brussels</wicri:regionArea><placeName><settlement type="city">Bruxelles</settlement><region nuts="2">Région de Bruxelles-Capitale</region></placeName></affiliation></author><author><name sortKey="Simn, C" sort="Simn, C" uniqKey="Simn C" first="C." last="Simn">C. Simn</name><affiliation wicri:level="1"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto Valenciano de Infertilidad (IVI), Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Espagne</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Human Reproduction</title><idno type="ISSN">0268-1161</idno><idno type="eISSN">1460-2350</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2009-04">2009-04</date><biblScope unit="volume">24</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="790">790</biblScope><biblScope unit="page" to="796">796</biblScope></imprint><idno type="ISSN">0268-1161</idno></series><idno type="istex">033DA12265BEEFC4242E6536902363F8C6BE7EA5</idno><idno type="DOI">10.1093/humrep/dep005</idno><idno type="ArticleID">dep005</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0268-1161</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">BACKGROUND Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS The IUCS device comprised a perforated silicone hollow tube. The study included 13 patients (<36 years) undergoing a first intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in three groups. In Group 1, 12 h after ICSI, sibling oocytes were assigned to IUCS or conventional in vitro culture. The device was retrieved on Day 1, and all zygotes were cultured in vitro till Day 5. In Group 2, fertilized oocytes were assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for blastomere biopsy and fluorescence in situ hybridization (FISH) and cultured until Day 5. The highest quality blastocysts were transferred on Day 5. RESULTS Fertilization and embryo development were comparable in the in vitro and IUCS arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in Group 3 revealed more normal embryos using the IUCS (P 0.049). Three clinical pregnancies and live births were obtained: two from the IUCS arm and one from the in vitro arm. CONCLUSIONS Our pilot study shows that this new IUCS appears to be feasible and safe, supporting normal fertilization, embryo development and normal chromosomal segregation. Furthermore, live births are possible after the transient presence of a silicone device in the uterus.Clinicaltrials.gov: NCT00480103.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Belgique</li><li>Espagne</li><li>France</li><li>Suisse</li></country><region><li>Basse-Saxe</li><li>Canton de Genève</li><li>Canton de Vaud</li><li>Région de Bruxelles-Capitale</li></region><settlement><li>Bruxelles</li><li>Genève</li><li>Hanovre</li><li>Lausanne</li></settlement><orgName><li>Université de Genève</li></orgName></list><tree><country name="Belgique"><region name="Région de Bruxelles-Capitale"><name sortKey="Blockeel, C" sort="Blockeel, C" uniqKey="Blockeel C" first="C." last="Blockeel">C. Blockeel</name></region><name sortKey="Devroey, P" sort="Devroey, P" uniqKey="Devroey P" first="P." last="Devroey">P. Devroey</name><name sortKey="Haentjens, P" sort="Haentjens, P" uniqKey="Haentjens P" first="P." last="Haentjens">P. Haentjens</name><name sortKey="Verheyen, G" sort="Verheyen, G" uniqKey="Verheyen G" first="G." last="Verheyen">G. Verheyen</name></country><country name="Suisse"><region name="Canton de Vaud"><name sortKey="Mock, P" sort="Mock, P" uniqKey="Mock P" first="P." last="Mock">P. Mock</name></region><name sortKey="Aebischer, P" sort="Aebischer, P" uniqKey="Aebischer P" first="P." last="Aebischer">P. Aebischer</name><name sortKey="Bouche, N" sort="Bouche, N" uniqKey="Bouche N" first="N." last="Bouche">N. Bouche</name><name sortKey="Bouche, N" sort="Bouche, N" uniqKey="Bouche N" first="N." last="Bouche">N. Bouche</name><name sortKey="Le Goff, Ph" sort="Le Goff, Ph" uniqKey="Le Goff P" first="Ph." last="Le Goff">Ph. Le Goff</name><name sortKey="Mock, P" sort="Mock, P" uniqKey="Mock P" first="P." last="Mock">P. Mock</name><name sortKey="Velasco, M" sort="Velasco, M" uniqKey="Velasco M" first="M." last="Velasco">M. Velasco</name></country><country name="France"><noRegion><name sortKey="Heyman, Y" sort="Heyman, Y" uniqKey="Heyman Y" first="Y." last="Heyman">Y. Heyman</name></noRegion></country><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Wrenzycki, C" sort="Wrenzycki, C" uniqKey="Wrenzycki C" first="C." last="Wrenzycki">C. Wrenzycki</name></region><name sortKey="Hffmann, K" sort="Hffmann, K" uniqKey="Hffmann K" first="K." last="Hffmann">K. Hffmann</name><name sortKey="Niemann, H" sort="Niemann, H" uniqKey="Niemann H" first="H." last="Niemann">H. Niemann</name></country><country name="Espagne"><noRegion><name sortKey="De Los Santos, M J" sort="De Los Santos, M J" uniqKey="De Los Santos M" first="M. J." last="De Los Santos">M. J. De Los Santos</name></noRegion><name sortKey="Fernandez Sanchez, M" sort="Fernandez Sanchez, M" uniqKey="Fernandez Sanchez M" first="M." last="Fernandez-Sanchez">M. Fernandez-Sanchez</name><name sortKey="Simn, C" sort="Simn, C" uniqKey="Simn C" first="C." last="Simn">C. Simn</name><name sortKey="Simn, C" sort="Simn, C" uniqKey="Simn C" first="C." last="Simn">C. Simn</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001129 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001129 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:033DA12265BEEFC4242E6536902363F8C6BE7EA5
|texte= An in vivo culture system for human embryos using an encapsulation technology: a pilot study
}}
| This area was generated with Dilib version V0.6.25. |  |